FITC抗体

异硫氰酸荧光素（FITC）是一种小分子半抗原，常用作荧光探针来标记蛋白和核酸分子和多糖和药物示踪。FITC性质稳定且1个抗FITC抗体能够同时结合3-4个FITC分子，可以通过免疫检测学利用FITC-抗FITC系统来提高抗原抗体反应的敏感性。抗FITC抗体在免疫检测，核酸杂交，免疫斑点技术及免疫组化等方面有着广泛应用。

FITC is a fluorochrome often conjugated or linked to an antibody in order to make a specific, fluorescent marker. When exposed to electromagnetic energy at a specific wavelength, fluorochromes are chemicals that will absorb the energy and emit a photon at a specific wavelength. FITC absorbs energy at 495 nm and emits a photon at 521 nm. Each fluorochrome has a different absorption and emission range, allowing for systematic detection of many fluorchrome-linked antibodies in one piece of tissue or sample. This is similar to labeling different parts of a cell with different colors and then using specialized glasses to look at just one color at a time.

Antibodies can be linked, or conjugated, to the FITC molecule to produce a FITC antibody. This FITC antibody can be used to directly or indirectly label a protein. When a FITC antibody directly labels a protein, it acts as the primary antibody, or an antibody in direct contact with the antigen. Indirect use of a FITC antibody occurs when it is used as a secondary antibody, binding specifically to a primary antibody. When a FITC conjugated antibody is used directly, the labeling procedure takes less time, but the labeling is not as strong. Indirect labeling takes an extra step, but the signal or label is amplified because several FITC-tagged secondary antibodies can attach to a single primary antibody.

待测抗原同荧光素FITC标记的抗体及酶（碱性磷酸酶）标记抗体结合形成“三明治”结构的复合物。随后加入连有抗荧光素抗体的磁微粒，通过荧光素抗体与荧光素的特异性结合使抗原抗体复合物链接在磁微粒上，在外加磁场中直接沉淀，将免疫反应形成复合物与未结合的其他物质分离。去上清后清洗沉淀的复合物，加入酶促化学发光底物，底物在酶作用下被催化裂解，形成不稳定的激发态中间体，当激发态中间体回到基态时便发出光子，形成发光反应。通过光量子阅读系统记录光子能量，并通过计算机处理系统将光能强度在标准曲线上转化为待测抗原的浓度，并报告结果。

本公司提供的FITC抗体，901035，灵敏高，特异性好，产量大且批间稳定，很好的应用于IVD领域化学发光，免疫层析和核酸POCT等。