FITC antibody

Fluorescein isothiocyanate (FITC) is a small molecule hapten that is commonly used as a fluorescent probe to label protein and nucleic acid molecules and to trace polysaccharides and drugs. The stable properties of FITCs and the ability of a single anti-FITC antibody to bind to 3-4 FITC molecules at the same time enable the use of the FitC-anti-FitC system through immunoassay to improve the sensitivity of antigen-antibody responses. Anti-fitc antibodies have been widely used in immunoassay, nucleic acid hybridization, immunospeckle technique and immunohistochemistry.

The antigen under test binds to a fluorescein FITC-labeled antibody and an enzyme (alkaline phosphatase) -labeled antibody to form a "sandwich" structure complex. Subsequently, magnetic particles with anti-fluorescein antibody are added, and the antigen-antibody complex is linked to the magnetic particle through the specific binding of fluorescein antibody and fluorescein. The antigen-antibody complex is directly precipitated in the external magnetic field, and the immunoreactive complex is separated from other unbound substances. After the supernatant is removed, the precipitated complex is cleaned and the enzymatic chemiluminescence substrate is added. The substrate is catalyzed and cracked under the action of the enzyme to form an unstable excited state intermediate. When the excited state intermediate returns to the ground state, it emits photons to form a luminescence reaction. The photon energy is recorded by the optical quantum reading system, and the light energy intensity is converted into the concentration of the antigen to be measured on the standard curve by the computer processing system, and the result is reported.